【摘要】 目的探讨培哚普利对链脲佐菌素(STZ)诱导的大鼠糖尿病模型肾小管间质中清道夫受体A(SR-A)表达的影响。方法将大鼠随机分为健康对照组、糖尿病组和培哚普利干预组。检测各组大鼠的血糖和尿清蛋白,行Masson染色观察肾脏形态学变化,应用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学检测SR-A mRNA及蛋白的表达。结果与健康对照组比较,糖尿病组大鼠血糖和24h尿清蛋白均显著上升;肾组织SR-A mRNA和蛋白表达均显著上调,培哚普利干预后,上述指标除血糖外均明显被抑制(P<0.05)。结论糖尿病大鼠肾小管间质SR-A表达增加,培哚普利对糖尿病大鼠肾脏有保护作用,其机制可能部分与下调肾小管间质SR-A表达有关。更多还原

【Abstract】 Objective To investigate the effect of an ACE inhibitor,perindopril,on the expression of SR-A in renal tubulointerstitium of diabetic rats.Methods Diabetes was induced in male Sprague-Dawley rats by injection with streptozotocin.The rats were then randomly divided into 3groups:normal control group;untreated diabetes mellitus group and diabetes mellitus group treated with perindopril.After a 24-week treatment,tubulointerstitial injury index was assessed with Masson′s trichrome sections.The expression of SR-A mRNA was detected by RT-PCR and the expression of SR-A protein in renal tubulointerstitium was detected by immunohistochemistry.Results The tubulointerstitial injury index,the expression of SR-A mRNA were significantly higher in the diabetes group than those in normal control group.Perindopril treatment not only attenuated the tubulointerstitial injury,but also reduced the overexpression of SR-A mRNA in diabetic rats.The expression of SR-A protein was most obvious in renal tubulointerstitium in diabetic rats,which was obviously attenuated by perindopril treatment(P<0.05).Conclusion The findings of the this study indicate that perindopril may have renoprotective effects on diabetic nephropathy via inhibiting the expression of SR-A in renal tubulointerstitium.更多还原