【摘要】 目的探讨炎症介质白细胞介素(IL)-6及IL-8在声门紧闭与开放状态下闭合性胸部撞击所致肺损伤组织中的表达。方法采用RT-PCR法检测声门紧闭与开放状态下,不同时相点胸部撞击伤侧与对照组肺组织IL-6和IL-8mRNA的表达。结果伤后4、8、12h声门紧闭组IL-6和IL-8mRNA的表达显著高于声门开放组(P<0.05)。伤侧肺组织中IL-6和IL-8mRNA表达各时相点显著高于与对照组(P<0.05)。对照组、声门紧闭组和声门开放组的肺组织两种细胞因子IL-8和IL-6mRNA的表达变化规律相似,表达明显上调。结论提示IL-6和IL-8可能在肺撞击伤后肺脏功能损害的发生、发展过程中发挥重要的作用。更多还原

【Abstract】 Objective To study the expression of IL-6and IL-8in the lung injuries induced by blunt chest trauma under the closure and open state of glottis.Methods The expressions of IL-6and IL-8mRNA were detected by RT-PCR in injuried lung tissues under the closed and open glottis states snd the control lung tissues at designed different time points.Results The expressions of IL-6and IL-8mRNA at 4,8,12 hin the closed glottis group were obviously higher than those in the open glttis group(P<0.05)and the expressions at designed various time points in the injured lung tissue were higher than those in the control lung tissues(P<0.05).The change rule of IL-6and IL-8mRNA expression in the control group,closed glottis group and open glottis group was similar,the expression was up-regulated.Conclusion IL-6and IL-8might play an important role in the occurrence and development of functional lesion in the injured lung by blunt chest trauma.更多还原