【摘要】 目的探讨非小细胞肺癌表皮生长因子受体(EGFR)基因突变与核苷酸切除修复交叉互补基因1(ERCC1)、胸苷酸合成酶(TYMS)mRNA表达的相关性。方法收集该科2013年2~12月符合入组条件的NSCLC患者97例,肿瘤组织标本由术中切取或穿刺获得。使用xTAG-液相芯片法检测EGFR(E18、E19、E20、E21)基因突变,使用分支DNA-液相芯片法检测ERCC1、TYMS mRNA表达水平,并分析EGFR基因突变与ERCC1、TYMS mRNA表达水平的相关性。结果 97例检测样本中,EGFR基因突变29例,突变率为30%(29/97),女性、无吸烟史、腺癌患者EGFR基因突变检出率较高(P<0.05)。EGFR突变与ERCC1mRNA表达有相关性(χ2=4.088,P<0.05),与TYMS mRNA表达无相关性(χ2=0.265,P>0.05)。结论NSCLC EGFR基因突变与ERCC1mRNA表达存在相关性,与TYMS mRNA表达无相关性。更多还原

【Abstract】 Objective To investigate whether EGFR gene mutations are correlated with the gene expression of ERCC1 and TYMS in non-small-cell lung cancer.Methods Collected February to December 2013 of non-small cell lung cancer(NSCLC)patients eligible for enrolled 97 patients,tumor tissue specimens obtained by intraoperative cut or puncture,Gene expression of ERCC1 and TYMS were determined by branched-DNA liquid chip,while somatic mutations in EGFR(E18,E19,E20,E21)gene were detected by xTAG-liquid chip;And analysis of EGFR gene mutation associated with ERCC1,TYMS mRNA expression.Results Totally29 cases of EGFR mutation were detected in all 97 specimens,with a mutation rate of 30%(29/97),and a relatively high detection rate was observed in female,adenocarcinoma and non-smoking patients(P<0.05).EGFR mutation was relevant to the expression of ERCC1(χ2=4.088,P<0.05),EGFR mutation was irrelevant to the expression of TYMS(χ2=0.265,P>0.05).Conclusion In NSCLC tissues,EGFR mutation is relevant to the expression of ERCC1 but irrelevant to the expression of TYMS.更多还原