【摘要】 目的研究γH2AX在男性不育患者精液中的表达水平,探讨γH2AX用于评价男性不育患者精子DNA双链断裂(DSBs)损伤程度的可行性。方法选取就诊于广西医科大学第一附属医院生殖医学研究中心、男性科门诊并确诊为男性不育患者27例(男性不育组),另外选取23例确认有生育能力的健康男性的精液样本作为对照组。通过PureSperm密度梯度离心法(DGC)优选精子,运用单向凝胶电泳技术(SCGE)和流式细胞术方法对健康男性、男性不育组患者的精液进行精子双链DNA损伤和γH2AX含量的测定和比较。结果男性不育组患者精子DSBs损伤程度和γH2AX表达含量均高于对照组(P<0.01),而经密度梯度离心法优选精子后,两组男性精子的DSBs损伤程度和γH2AX表达含量均显著下降(P<0.01)。结论γH2AX在男性不育患者精子中的含量明显高于健康男性,可作为检测精子DNA双链断裂损伤程度的一个新的标志物。更多还原

【Abstract】 Objective To investigate whetherγH2AX could be a useful biomarker for evaluating the DNA double-stranded.Methods Semem samples in case group were from 27 infertile males who were diagnosed in Andriatrics department or reproductive centre in the First Affiliated Hospital of Guangxi Medical University.The other semen samples were from 23 healthy donors with fertility as comparison.The levels ofγH2AX were detected by flow cytometry.Single cell gel electropherosis(SCGE)was applied to assess the level of DSBs of sperm.Density gradient centrifugation(DGC)was applied to optimized spermatozoa.Results TheγH2AX levels and the DSBs of the sperm of the infertile subjects were significantly higher than those of healthy males(P<0.01),and the levels ofγH2AX and the DSBs of sperm significantly decreased in two groups by DGC(P<0.01).Conclusion The level of spermatozoaγH2AX is higher in male infertility patients than in healthy donors with fertility,which might be a useful biomarker for evaluating DSBs of sperm.更多还原