【摘要】 目的探讨一种将中药血清药理学和电生理学技术结合的方法,并将该方法运用到中药丹参的有效成分丹参酮ⅡA磺酸钠(DS-201)的舒血管机制研究。方法采用醇提法提取丹参有效成分,将该提取液给予小鼠灌胃,经眼球采血得到小鼠血清,用高效液相色谱法(HPLC)测定小鼠血清中DS-201的含量,并将该含药血清应用于单通道膜片钳实验,研究其对猪冠状动脉平滑肌细胞(PCASMCs)大电导钙激活钾通道(BKCa)的作用。结果含DS-201血清浓度线性范围是0.73~1.91μg/mL(r=0.997 7),回收率是99.85%~101.47%,血药浓度为(7.32±4.25)μg/mL;单通道膜片钳实验结果显示小鼠含DS-201血清对PCASMCs的BKCa有激活作用,但差异无统计学意义(P>0.05)。结论该研究建立的提取丹参有效成分丹参酮的醇提法简单、实用、可靠,且测定DS-201含量的HPLC方法精确;应选取高质量丹参和(或)提高丹参在体内的生物利用度,才能更好地将血清药理学和电生理学技术结合在一起。更多还原

【Abstract】 Objective To discuss a method combining serum-pharmacology and electrophysiology technology,and to research the mechanism of dilating porcine coronary artery of sodium tanshinoneⅡ-A sulfonate(DS-201).Methods To give mice intragastric administration solution and measure DS-201 concentration in mice serum,and apply the serum to single channel patch to research its effect on big conductance calcium-activated potassium channels(BKca)in porcine coronary artery smooth muscle cells(PCASMCs).Results The linear range of concentration containing DS-201 serum was 0.73 to 1.91μg/mL(r=0.997 7),the recycle rate was99.85%-101.47%,and the concentration was(7.32±4.25)μg/mL;the result indicates that serum containing DS-201 has activation effects on BKCain PCASMCs,while there was no statistical significance(P>0.05).Conclusion The establishment method of the alcohol extraction of Danshen is useful and reliable.The HPLC method of measuring DS-201 concentration is precise.Choosing higher quality drugs or raising bioavailability can improve combination of the serum pharmacology and electrophysiological technique.更多还原