Based on the production and the enzymatic activity of recombinant proteins from Pichia pastoris with different fermentations,the fermentation factors of human butyrylcholinesterase were optimized.On the basis of single factor experiment,the conditions were optimized by response surface methodology,those were:4.8d,13.90% of methanol and 2.1% of glycerol.The yield of human butyrylcholinesterase was 368.2mg/L.The study provided a reference for industrial production of human butyrylcholinesterase.
【基金】
北京市农业科技项目(201301010)
【更新日期】
2015-11-20
【分类号】
TQ925
【正文快照】
TIAN Jingjing1,CHEN Xiangning1*,XIE Yuanhong1,XIAO Zhiyong2(1.College of Food Science and Engineering,Beijing University of Agriculture/Beijing Key Laboratoryof Harmful Microorganisms and Pesticide Residues Detection and Control on Agricultural Product,Be
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
