【摘要】 以α-氰基丙烯酸异丁酯和α-氰基丙烯酸正辛酯为原料,泊洛沙姆188为表面活性剂,通过乳化法制备了聚(α-氰基丙烯酸酯)共聚物微球,然后通过共聚物微球对溶菌酶的吸附,制备了共聚物载药微球。考察了表面活性剂质量分数对微球粒径的影响,并就共聚物和药物的质量分数及吸附时间对微球粒径、载药率和负载率的影响进行了研究。结果表明:共聚物空白微球适宜的制备条件为,共聚物质量分数0.8%(α-氰基丙烯酸正辛酯与α-氰基丙烯酸异丁酯的物质的量比为1∶1),泊洛沙姆188质量分数1.0%,搅拌速度800r/min,体系pH2.5,反应时间3h;空白微球在pH7.0条件下进行溶菌酶吸附,在溶菌酶质量分数0.08%、吸附3h时所得的共聚物载药微球性能最佳,且所得的载药微球具有缓释特性。更多还原

【Abstract】 Poly(α-cyanoacrylate) copolymer microspheres have been prepared by emulsion polymerization with α-isobutyl cyanoacrylate(i BCA) and α-octyl cyanoacrylate(OCA) as carrier materials,and poloxamer 188 as emulsifier.Lysozyme was then adsorbed on the surface of the poly(α-cyanoacrylate) copolymer microspheres to give drug-loaded microspheres.The effects of the emulsifier,copolymer or drug mass fraction and the adsorption time on the size of the microspheres,the encapsulation efficiency,and the drug loading were investigated in detail.The optimum conditions for the preparation of the poly(α-cyanoacrylate) copolymer microspheres were found to be 0.8% iBCA and OCA in a mole ratio of 1∶ 1,1.0% mass fraction of poloxamer 188,and pH2.5,stirring speed 800r/min,and a particle formation time of 3h.Lysozyme was subsequently adsorbed on the copolymer microspheres for 3h at pH7.0 with a lysozyme concentration of 0.08%.The drug-loaded microspheres had good slow-release characteristics.更多还原