【摘要】 以34份鸢尾属园艺品种为试材,采用ISSR分子标记方法,研究鸢尾属园艺品种的遗传特性及亲缘关系。结果表明:10个ISSR随机引物检测到153个多态位点,多态性条带比例为100%,表明供试材料具有丰富的遗传多态性。各品种之间的DICE遗传相似系数在0.148~0.657之间,平均相似系数为0.365。由聚类分析可知,当遗传系数为0.354时,可将供试材料分为4个品种群。其中Ⅰ群有21个品种,全部为须毛状附属物亚属的德国鸢尾。Ⅱ群有4个品种,包括无附属物亚属的西伯利亚鸢尾和2个花菖蒲品种。Ⅲ群有4个品种,包括2个花菖蒲品种以及2个从荷兰引进的德国鸢尾。Ⅳ群为无附属物亚属的路易斯安那鸢尾品种群。主坐标分析所反映的关系和UPGMA聚类图相似。更多还原

【Abstract】 Inter-simple sequence repeat(ISSR)was used to detect the genetic diversity among 34 horticultural cultivars of Iris.The results showed that 10 primers produce 153 clear,polymorphic and steady bands.All of bands were polymorphic(100%),which indicated that the genetic diversity of Iris L.was rich.The genetic similarity coefficient among the accessions ranged from 0.148 to 0.657,and the average was 0.365.At the point of the similarity coefficient 0.354,all the accessions might be separated into four groups.The group I consisted by the cultivars of I.germanica.The group II had tow cultivars of I.sibiricaand tow of I.ensata.In group III,towI.ensataand tow of germanica from Holland were included.The IV group was made up with cultivars of I.Louisiana.The results of principal coordinates showed similarity to the UPGMA dendrogram.更多还原