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白灵菇杂交子的筛选及鉴定
Selection and Identification of Hybrid Strain of Pleurotus eryngii var.tuoliensis
【摘要】 以白灵菇2个品种"中农1号"和"中农翅鲍"为亲本,采用镜检、拮抗、酯酶同工酶及原基诱导等方法,研究了150对杂交组合菌株特性。结果表明:150对杂交组合菌株中,99个菌株生长较快,有锁状联合,75个与2个亲本有明显拮抗线,且具与2个亲本菌株的典型酯酶同工酶条带,确定是杂交菌株,其中50个杂交子具有出菇能力。
【Abstract】 Taking two different Pleurotus eryngii var.tuoliensis strains as parents(‘Zhongnong No.1’and‘Zhongnong Chibao’),the proterties of 150 cross combinations were studied by microscopy,antagonism,esterase isozyme and primordium induction.The results showed that 99 of 150hybrid strains were with clamp-connection,75 strains had obvious antagonism with two parents and had new enzyme bands and complementary enzyme bands,50 strains had primordium formation in PDA plate.
【关键词】 白灵菇;
杂交子;
拮抗;
酯酶同工酶;
原基形成;
【Key words】 Pleurotus eryngii var.tuoliensis; hybrid strain; antagonistic test; isozyme analysis; primordium formation;
【Key words】 Pleurotus eryngii var.tuoliensis; hybrid strain; antagonistic test; isozyme analysis; primordium formation;
【基金】 河北省现代农业产业技术体系食用菌产业创新团队建设专项资金资助项目
- 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2015年09期
- 【分类号】S646
- 【被引频次】1
- 【下载频次】154