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分光光度法测定红花软胶囊中总黄酮的含量

Determination of Total Flavonoids in Safflower Soft Caplsule by UV sepectrophotometry

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【作者】 翟晓茹; 张红兵; 李永东; 张燕;

【Author】 Zhai Xiaoru;Zhang Hongbing;Li Yongdong;Zhang Yan;Hua Wei Pharmaceutial company of China Institute for radiation protection;

【机构】 中国辐射防护研究院华卫药业;

【摘要】 目的:建立红花软胶囊中总黄酮含量的检测方法。方法:采用UV法,在波长360nm处测定红花软胶囊中总黄酮的含量。结果:用芦丁作对照,在4.657~23.285μg/m L范围内线性关系良好,r=1.0000,平均回收率为97.10%(RSD=1.1%,n=9)。结论:该方法准确、灵敏、重现性好,可用于红花软胶囊中总黄酮含量的测定。

【Abstract】 Objective: To establish a method to determine the content of total flavonoids in Safflower Soft Caplsule. Methods : The content of total flavonoids was determined by UV at 360 nm. Results: The linear range was between4.657 ~23.285μg/m L with correlation coefficient of r=0.9995. The average recovery(n=5)and RSD were 97.10% and 1.1% respectively. Conclusion: The established method is accurate and reliable with good reproducibility. It can be used to control the quality total flavonoids in Safflower Soft Caplsule.

【基金】 新疆自治区重大专项(编号:200733146-1)
  • 【文献出处】 北方药学 ,Journal of North Pharmacy , 编辑部邮箱 ,2015年09期
  • 【分类号】R286.0;O657.32
  • 【被引频次】2
  • 【下载频次】145
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