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安徽省赤练蛇野生群体遗传多样性分析

Analysis on Population Genetic Diversity of the Natural Chinese of Dinodon rufozonatum

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【作者】 鲍方印肖明松王松华何庆元

【Author】 BAO Fang-yin;XIAO Ming-song;WANG Song-hua;HE Qing-yuan;College of Life Science,Anhui Science and Technology University;

【机构】 安徽科技学院生命科学学院

【摘要】 利用mt DNA控制区(D-Loop)序列作为分子标记,对安徽省野生赤链蛇6个野生群体14个个体的遗传结构进行了分析。结果表明,赤链蛇D-Loop序列长690 bp,6个种群共检测到变异位点20个,占全部序列的3.42%,14个个体共检测到6种单倍型。采用邻接法(NJ法)对所有单倍型进行系统发育树构建,结果表明:安徽省野生赤链蛇可以分为3支,大别山区和皖南山区各1支,淮北、江淮丘陵及沿江为1支。

【Abstract】 In this study,the mitochondrial DNA control region( D- Loop) was used to analyze genetic diversity and structure of 6 natural populations of Dinodon rufozonatum collected from Huangzangyu,Tushan,Huangpushan,Tiantangzhai,Jinshan and Hongcun separately. The results showed the length of this region( D- loop) contained 690 bp nucleotides and the A,C,T and G contents were 32. 3%,24. 8%,27. 9% and 15. 0% respectively. 20 nucleotide sites and 6 haplotypes were found in 6 wild populations of Dinodon rufozonatum. Molecular phylogenetic tree constructed by NJ method showed individuals from the different populations did not cluster together. These results suggested wild Dinodon rufozonatum from Anhui could be divided into three branches,which were the Dabie Mountains and mountainous area of Southern Anhui,Huaibei,hilly areas between Yangtze River and Huaihe River and along the Yangtze River respectively.

【关键词】 赤链蛇mtDNAD-Loop遗传差异
【Key words】 Dinodon rufozonatummt DNAD-LoopGenetic difference
【基金】 安徽省高校自然科学研究重点项目(KJ2010A080)
  • 【文献出处】 安徽科技学院学报 ,Journal of Anhui Science and Technology University , 编辑部邮箱 ,2015年05期
  • 【分类号】S865.32
  • 【下载频次】76
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