In order to clarify the relationship between the toxicity of insecticides and its determination method,the paper selected the aphid virulence as experimental objects using the immersion,isolated spray method and continuous infusion method for the determination of thiamethoxam and dinotefuran.Results showed that the virulence of thiamethoxam impregnation method and continuous immersion method of broad bean aphid was significantly higher than that in vitro spray method.Virulence dinotefuran impregnation on th...