【摘要】 目的:探讨针灸推拿体外压刺激对血管内皮细胞ET-1、PGI₂、IFN-β和TNF-α的影响。方法:体外培养血管内皮细胞,平分为空白对照组、低压力力学刺激组和高压力刺激组,分别予以0kPa、100kPa和200kPa压力刺激,检测细胞外培养液血管活性物质ET-1、PGI₂、IFN-β和TNF-α含量。结果:低、高压力刺激组PGI₂合成释放量均显著少于空白对照组（P<0.01）,高压力刺激组ET-1合成释放多于空白对照组（P<0.05）;高压力刺激组IFN-β合成释放量显著多于空白对照组（P<0.05）,低、高压力刺激组TNF-α合成释放量均多于空白对照组（P<0.05）。结论:压力刺激调节血管内皮细胞血管舒缩活性物质ET-1、PGI₂合成释放,促进具有免疫调节功能的细胞因子IFN-β、TNF-α合成释放,可能是针灸推拿发挥“运气血、扶助正气”作用的细胞生物力学原理之一。更多还原

【Abstract】 Objective:To investigate the effects of acupuncture and massage on ET-1,PGI₂,IFN-β and TNF-α of vascular endothelial cells.Methods:The vascular endothelial cells were cultured external and divided into blank control group,low pressure mechanical stimulation group and high pressure stimulation group,respectively with OkPa,100 kPA,200kPa pressure stimulation,content of vasoactive substance ET-1,PGI₂,IFN-β and TNF-αof extracellular nutrient solution was detected.Results:The synthetize and release of PGI₂ in low and high pressure stimulation group were both significantly less than that in blank control group（P<0.01）,and ET-1 of high pressure stimulation group was more than that of blank control group（P<0.05）;IFN-β of high pressure stimulation group was significantly more than that of blank control group（P<0.05）,TNF-α of low and high pressure stimulation group was more than that of blank control group（P<0.05）.Conclusion:Pressure stimulation regulates the synthetize and release of vasomotor active substances ET-1 and PGI₂ of vascular endothelial cells,and promotes the synthetize and release of cytokines IFN-β and TNF-α with immune regulating function,which could be one of biomechanical principles of acupuncture and massage playing a role of "carrying qi and blood,strengthening genuine qi".更多还原