[Objective] To study the effects of different water content on preservation of Iris sanguinea seed.[Method]There is no denying that liquid nitrogen cryopreservation is an ideal way to preserve germplasm for a long time. When using seeds as saving materials,the optimal moisture contents and thawed are very important to the effect of cryopreservation. This study uses the wild Iris sanguinea seeds for the experimental materials which are grow in cold places. We get the seeds of different water contents with th...