[Objective] The aim is to study the physical and chemical properties of three substrate media and their effects on banana tissue culture seedlings growth. [Method] Three culture media as coconut coir,peat soil and red loam were detected for their physical-chemical properties,and different effects on banana tissue culture seedlings growth were compared. [Result]The bulk density is significantly smaller in coconut coir and peat soil than that of red loam. Their total porosity is 82. 6% and 77. 0%,respectively...