[Objective] The high performance liquid chromatographic method was developed for determination of chlorogenic acid and galuteolin simultaneously in different honeysuckle.[Method] Kromasil-C18 Column(250 × 4.6 mm,5 μm) was used for gradient elution with a mobile phase of mixture of methanol-1% phosphoric acid solution at a flow rate of 1.0 ml/min with the detection wavelength of 350 nm at room temperature.[Result] The calibration curve was linear in the range of 0-1 mg/ml for chlorogenic acid(r = 0.999 1),an...
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