[Objective] In order to select the optimal liquid medium suitable for mycelium of Hypsizigus marmoreus(Peck) Bigelw.[Method] Adopting mycelial biomass of Hypsizigus marmoreus(Peck) Bigelw as major index,firstly the optimum carbon and nitrogen source of Hypsizigus marmoreus(Peck) Bigelw were used in the single factor test,and then carrying out the orthogonal test for the different concentrations of carbon and nitrogen source. Further,filtrating the optimum inorganic salt and VB1 were used in the single facto...