[Objective]The aim was to study the development of Hippeastrum vittatum by callus induced using bulbs as explants. [Method]Callus were induced using bulbs as explants then the protocorm was developed and regeneration plants were established. [Result]The results showed that the optimal medium for callus induction was MS + 6-BA 2. 0 mg / L + NAA 1. 0 mg / L,the callus induction rate of bulbs was the highest,the induction rate reached 80%; medium to induce proliferation of protocorm like bodies was MS + 6-BA 1...