[Objective] With Swainsona salsula seeds and different parts of seedlings as explants,aseptic processing and culture was conducted,methods to control the rate of bacterial contamination were explored.[Method]Using MS as basic medium,through different types and concentrations of disinfection reagent ratio,time,the effects on explant primary culture contamination rate were discussed. [Result] The results showed that the seeds of wild salsula peeled with 0. 1% mercuric chloride is better,better disinfection ti...
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