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百合各株系中秋水仙碱提取方法优化及含量测定

Determination of colchicine and Optimization of extraction method in Lilium brownii

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【作者】 姚红锐尚靖柳军卜长秀

【Author】 YAO Hong-rui;SHANG Jing;LIU Jun;BU Chang-xiu;The first affiliated hospital Anhui University of Chinese Medicine;New drug screening center of China Pharmaceutical University;Anhui Hospital of Armed Police Corps;

【机构】 安徽中医药大学第一附属医院药学部中国药科大学新药筛选中心武警安徽省总队医院

【摘要】 目的:对百合各株系中秋水仙碱进行提取方法优化并建立含量测定方法。方法:采用正交实验设计和HPLC法进行提取工艺优化和含量测定。结果:百合各株系中秋水仙碱含量普遍高于对照组,对提取条件进行探讨,确定了最佳工艺条件)。结论:优化及测定方法准确可靠,可用于选育优良品种的质量控制。

【Abstract】 Objective:To establish a method for the determination of colchicine and optimize the extraction methods in Lilium brownii. Methods: Extraction process was optimized and determined through Orthogonal experimental design and HPLC method. Results:The Contents of colchicine was higher than the control group.The extraction conditions were discussed,and the optimum process conditions were determined. Conclusion: The method was precise and can be used for quality control of fine variety bredding.

【关键词】 百合秋水仙碱提取方法含量测定
【Key words】 Lilium brownicolchicineextraction methoddetermination
  • 【文献出处】 中医药临床杂志 ,Clinical Journal of Traditional Chinese Medicine , 编辑部邮箱 ,2015年05期
  • 【分类号】R284
  • 【被引频次】5
  • 【下载频次】737
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