【摘要】 目的:研究大鼠孕期和哺乳期暴露2,4-二氯苯氧乙酸(2,4-D)对子代发育及脑组织脂质过氧化的损伤作用。方法:Wistar大鼠于受孕后第2天开始经口灌胃染毒2,4-D(0、25、50和100 mg/kg)直至仔鼠出生后第21天,每天1次,连续42 d。期间检测仔鼠生理及早期神经行为发育指标。断乳1周后处死仔鼠检测脑组织丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量及谷胱甘肽过氧化物酶(GSH-Px)的活力。结果:与对照组相比,100 mg/kg剂量组仔鼠体质量从出生后14 d开始降低,50 mg/kg剂量组仔鼠体质量从出生后21 d开始降低,差异均有统计学意义(P<0.05),而其他各生理发育指标差异均无统计学意义(P>0.05)。神经行为测试中100 mg/kg剂量组仔鼠断崖回避、空中翻正及听觉惊愕的阳性发生率均明显低于对照组(P<0.05),出现神经行为发育迟缓。50和100mg/kg剂量组仔鼠脑组织中MDA含量升高,100 mg/kg剂量组仔鼠脑组织GSH含量及GSH-Px的活性下降,与对照组相比差异均有统计学意义(P均<0.05)。结论:大鼠孕期和哺乳期暴露2,4-D致子代神经发育迟缓可能与2,4-D引起脑组织脂质过氧化损伤有关。更多还原

【Abstract】 OBJECTIVE: To study the effects of 2,4-dichlorophenoxyacetic acid(2,4-D) on early neurobehavior development and oxidative stress in offsprings. METHODS:Pregnant Wistar rats were treated with 2,4-D by gavage,in doses of 0,25,50,100 mg/kg once a day from gestation day 2 until postnatal day 21. Then the early physiological and neurobehavioral indexes in the offsprings were measured. The levels of MDA,GSH and the GSH-Px activities in brain tissues of newborn rat were determined. RESULTS:The weights of offspring in the 100 mg/kg 2,4-D group were lower than in control after postnatal day 14(P<0.05) and the weights of offspring in the 50 mg/kg 2,4-D were lower than in control after postnatal day 21(P<0.05). The physiologic markers including eye opening,pinna detachment,hair growth,tooth growth etc in all treated groups were not affected in contrast to the control group,cliff avoidance,mid-air righting and acoustic startle in the 100 mg/kg 2,4-D group were delayed(P<0.05). The level of GSH and GSH-Px activities decreased in the 100 mg/kg 2,4-D group,while the level of MDA was elevated in the 50 and 100 mg/kg 2,4-D groups. CONCLUSION:Early neurobehavioral development of offspring rats could be impaired by 2,4-D due to its disturbance to the homeostasis of oxidation and anti-oxidation system.更多还原