【摘要】 目的:探讨人L-02肝细胞经三氯乙烯(TCE)染毒后SET相互作用蛋白e EF1A1、e EF1A2和DDB1 m RNA表达水平的变化。方法:取对数生长期的L-02肝细胞,暴露于不同浓度TCE(1.0、2.0、4.0、8.0 mmol/L)中,以DMSO为溶剂对照。24 h后,采用实时荧光定量PCR检测e EF1A1、e EF1A2和DDB1 m RNA表达水平。结果:与对照组相比,不同浓度的TCE均能够使L-02肝细胞e EF1A1、e EF1A2和DDB1 m RNA表达水平发生明显变化。其中,DDB1和e EF1A2 m RNA表达水平在低浓度(1.0 mmol/L)TCE暴露下显著升高,而随着TCE浓度的升高其m RNA表达水平显著降低(P<0.05或P<0.01)。e EF1A1 m RNA表达水平随着TCE浓度的增加而升高(P<0.05)。结论:TCE染毒可诱发L-02肝细胞中SET相互作用蛋白的m RNA表达水平发生改变。更多还原

【Abstract】 OBJECTIVE: To research the effects of trichloroethylene on the RNA expressions of SET-associated proteins including e EF1A1,e EF1A2 and DDB1 in L-02 cells. METHODS:L-02 cells,being in the logarithmic growth phase,were treated with different concentrations( 1.0,2.0,4.0,8.0 m mol/L) of t richloroethylene for 2 4 h,and DMSO were used as control. The mRNA levels of eEF1A1, eEF1A2 and DDB1 were analyzed with qPCR. RESULTS:The results showed that mRNA levels of DDB1 and e EF1A2 were significantly up-regulated under low concentration of trichloroethylene(1.0 mmol/L) and were significantly down-regulated with increasing concentrations of trichloroethylene. The mRNA levels of eEF1A1 were significantly up-regulated with increasing of trichloroethylene concentration. CONCLUSION:Exposure of trichloroethylene could alter mRNA expression of SET-associated proteins in L-02 cells.更多还原