【摘要】 目的:复制氧化性低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)诱导的人脐静脉内皮细胞(Human Umbilical Vein Endothelial Cells,HUVECs)氧化损伤模型,并探讨熊果酸(Ursolic Acid,UA)对其保护作用。方法:以体外培养的HUVECs为实验对象,采用不同浓度(5,10,15,20μM)熊果酸及阳性对照药物维生素E预处理细胞16 h,再加入100μg/ml的ox-LDL培养24 h造成细胞氧化损伤。CCK-8检测细胞存活率,酶联免疫法检测各组细胞培养上清液过氧化氢(H2O2)、一氧化氮(NO)、丙二醛(MDA)含量,超氧化物歧化酶(SOD)活力以及各组细胞裂解液中一氧化氮合成酶(NOS)活力;采用SPSS 17.0软件对实验数据进行统计学分析。结果:与正常组相比,模型组细胞存活率、NO、SOD、NOS水平明显降低,MDA、H2O2水平明显增加;与模型组比较,熊果酸在5~20μM范围内,呈剂量依赖性提高细胞存活率,上调NO、SOD、NOS水平,并下调MDA、H2O2水平。结论:100μg/ml的ox-LDL能够诱导HUVECs发生氧化损伤,熊果酸能够通过抗氧化发挥保护作用,其机制可能与维持NOS、SOD活性、减少MDA、H2O2生成等有关。更多还原

【Abstract】 Objective: To investigate the protective effects and mechanisms of ursolic acid on damaged umbilical vein endothelial cells( HUVECs)induced by ox-LDL. Methods: HUVECs were administered with different concentrations( 5,10,15,20 μM) of UA for 16 h. Then they were treated with 100 ? g /ml ox-LDL for 24 h to duplicate an oxidative injury model. The cell viability of HUVECs was detected by CCK-8. The content of hydrogen peroxide( H2O2),nitric oxide( NO),malondialdehyde( MDA) and the activity of superoxide dismutase( SOD) and nitric oxide synthase( NOS) were detected by enzymic method. The experimental data was statistical analysised by SPSS 17. 0. Results: Compared with the control group,the cell viability,NO,SOD,NOS levels in model group were significantly declined while the MDA,H2O2 levels were significantly increased( P < 0. 05). Compared with the model group,UA could improve the cell viability,increase the NO,SOD,NOS levels and lower the MDA,H2O2 levels in a dose-dependent manner. Conclusion: 100 μg /ml ox-LDL could induce oxidative damage of HUVECs,and UA could play a protective role through its anti-oxidative effect. This may be related to its effect of maintaining NOS,SOD activity,reducing MDA,H2O2 generation and so on.更多还原