【摘要】 目的:探讨白术多糖的毒性及抗神经细胞缺氧性凋亡作用。方法:研究分为正常对照组、凋亡阳性组、白术多糖0.025g/L组、白术多糖0.05g/L组、白术多糖0.1g/L组、白术多糖0.25g/L组。采用"Neurobasal+B27"体外神经细胞无血清培养,免疫细胞化学鉴定神经细胞,MTT测定药物毒性,AnnexinV/PI荧光双染流式细胞仪及Hochest33342荧光染色检测细胞凋亡。结果:白术多糖在1.0g/L之内预处理神经细胞对其无毒性作用。白术多糖在0.025g/L~0.05g/L范围显著地改善缺氧对神经细胞生长的抑制,在0.025g/L~0.25g/L范围内显著地抑制缺氧复氧诱导的神经细胞早期凋亡。结论:白术多糖在一定浓度范围内具有抗缺氧复氧诱导的细胞凋亡作用。更多还原

【Abstract】 Objective: To explore the effect of Atractylodes macrocephalaon polysaccharides( AMPS) on cytotoxicity and preventing the neuron apoptosis indued by hypoxia. Methods: The study was divided into normal control group( group C),apoptosis positive group induced by hypoxia( group A),experiment 1 group treated by 0. 025g /L AMPS( AMPS1),experiment 2 group treated by 0. 05g /L AMPS group( AMPS2),experiment3 group treated by0.1g/L AMPS group( AMPS3),experiment4 group treated by0.25 g/L AMPS group( AMPS4).Neurons were cultured by Neurobasal + B27 in serum-free in vitro. Neurons were identified by immunocytochemistry. Drug toxicity was determined by MTT assay,Neuron apoptosis was examined by Annexin V- FITC /PI in flow cytometry and Hoechst 33342 fluorescent staining under fluorescence microscope. Results: The AMPS was non-toxic for neurons pretreated within 1. 0 g /L. The AMPS significantly prevented the inhibition of neuron growth from hypoxia from 0. 025g /L to 0. 05g /L,( P < 0. 05). Early apoptosis of neurons induced by hypoxia-reoxygenation was significantly inhibited by AMPS from 0. 025g /L to 0. 25g /L( P < 0. 05). Conclusion: The AMPS has an effect on inhibitting early apoptosis of neurons induced by hypoxia-reoxygenation in a certain concentration range.更多还原