【摘要】 目的:探讨玉屏风散对变应性鼻炎细胞因子活性的影响。方法:将SD大鼠60只随机分为空白组、模型组、阳性药酮替芬组和玉屏风散12、6及3g/kg剂量组,每组10只;除空白组外,其余各组联合卵蛋白及氢氧化铝复制AR(Allergic rhinitis,AR)大鼠模型。给药组大鼠分别灌胃给予0.46g/kg酮替芬及12、6及3g/kg的玉屏风散,1次/天,观察动物鼻痒、喷嚏及流涕等症状并计分;取鼻腔灌洗液,用蛋白印迹法测定其肥大细胞中IL-8及IL-10的表达水平;实验结束取血清采用酶联免疫法测定其白介素-8(IL-8)及白介素-10(IL-10)的含量;取鼻粘膜采用HE染色行病理组织检测。结果:12g/kg、6g/kg玉屏风散玉屏风散能显著改善AR大鼠的过敏症状;能显著降低模型动物血清IL-8而升高IL-10的含量,能显著升高鼻腔灌洗液中肥大细胞IL-10的表达水平,而降低IL-8的表达水平,差异有统计学意义;12g/kg玉屏风散玉屏风散能显著改善模型大鼠鼻粘膜的病理改变。结论:玉屏风散治疗变应性鼻炎的机理,可能是通过抑制炎性细胞因子和激活抗炎性细胞因子水平的"双向调节"机制实现的。更多还原

【Abstract】 Objective: To probe the effect of Yupingfeng Powder to the activity of cytokines of allergic rhinitis( AR). Methods: Healthy rats were divided into six groups which were control group,model group,positive group,high dose,middle dose and low dose of Yupingfeng powder respectively and each group had ten rats. The rats of each group were administrated by aluminium hydroxide [Al( OH)3]and ovalbu-min( OVA) to copy the allergic rhinitis rats expect for the control group. And each group were administrated the corresponding medicine by oral and the model group were administrated the equivoluminal distilled water. Observed and scored such allergic syndromes as rhinocnesmus,sneeze and rhinorrhoea of the rats; determined of the content of interleukin-8( IL-8) and interleukin-10( IL-10) in the serum of rats by euzymelinked immunosorbent assay( ELSIA); test the expression level of IL-8 and IL-10 in the nasal lavage fluid( NLF) of rats by western blotting( WB); detected the pathological changes in the nasal mucosa tissue by hematoxylin-eosin( HE) staining. Results: The dose of12g /kg and 6g /kg of Yupingfeng powder could improve the allergic syndromes; could decrease the content of IL-8 but increase the content of IL-10 in the serum of AR rats and the difference between the administrated group and the model group was significant( P < 0. 05,P < 0. 01);could increase the expression level of IL-10 but decrease the expression level of IL-8 in the NLF and the difference between the administrated group and the model group was significant( P < 0. 05,P < 0. 01); the dose of 12g /kg of Yupingfeng powder could improve the pathological changes of nasal mucosa tissue. Conclusion: The mechanism of Yupingfeng powder on curing the AR maybe affect through the two-way regulatory which it could inhabit the inflammatory cytokine but activate the anti-inflammatory cytokine.更多还原