【摘要】 目的利用高效液相色谱(HPLC)法,建立颈眩口服液中天麻素和芍药苷的含量测定方法。方法采用Hypersil ODS2 C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸梯度洗脱,天麻素与芍药苷的检测波长分别为220 nm和230 nm,柱温为室温。结果天麻素在0.116~1.160μg进样量范围内线性关系良好,平均加样回收率为101.11%,RSD为2.51%;芍药苷在0.05~1.00μg进样量范围内线性关系良好,平均加样回收率为98.27%,RSD为1.64%。结论该方法能简便、灵敏、准确的测定颈眩口服液中天麻素和芍药苷的含量,可用于该制剂的质量控制。更多还原

【Abstract】 Objective To establish a method for simultaneous determination of gastrodin and paeoniflorin by high performance liquid chromatography in Jingxuan oral liquid. Methods The analysis was performed on Hypersil ODS2C18(250 mm×4.6 mm,5 μm)column. The detection was with a mobile phase of acetonitrile-0.1 % phosphonic acid solution at the flow rate of 1 m L·min-1by gradient elution. The wavelength of detector was set at 220 nm for gastrodin and 230 nm for paeoniflorin, respectively. Results The calibration curve of gastrodin and paeoniflorin showed good linear relationship in the range of 0.116~1.160 μg and 0.05~1.00 μg, respectively. And the average recovery was101.11 %(RSD=2.51 %,n=6)for gastrodin,98.27 %(RSD=1.64 %,n=6)for paeoniflorin. Conclusion The method is simple,sensitive and accurate to determine the content of gastrodin and paeoniflorin in Jingxuan oral liquid,and is suitable for the quality control of Jingxuan oral liquid.更多还原