【摘要】 目的:研究3,5-二硝基水杨酸(DNS)法测定羟乙基淀粉还原端的条件。方法:以葡萄糖做标准曲线来测定羟乙基淀粉还原端的含量,研究DNS试剂的用量、加热温度、加热时间及反应后放置时间对测定结果的影响。结果:DNS法测定羟乙基淀粉的最佳反应条件是:DNS试剂用量0.8 ml,反应温度85℃,反应时间5 min,显色定容后于540 nm的波长处测定吸光度。结论:本方法操作简单、准确,重复性好,可用于羟乙基淀粉还原端的测定。更多还原

【Abstract】 Objective: To explore the determination conditions for reducing end of hydroxyethyl starch by DNS spectrophotometry.Methods: The reducing end of hydroxyethyl starch was determined using the standard curve of glucose solutions. The effects of DNS reagent with different volume,heating temperature,heating time and standing time after reaction on the determination were investigated. Results: The optimal determination conditions were as follows: the DNS volume was 0. 8 ml,the reaction temperature was 85 ℃,the reaction time was 5 minutes,and the colored solution was determined at the wavelength of 540 nm. Conclusion: The method is simple and accurate with good reproducibility,which can be used to determine reducing end of hydroxyethyl starch.更多还原