【摘要】 目的研究上海市西南地区成人病毒感染性腹泻患者的病原学特点。方法采用逆转录荧光定量-PCR方法进行病毒检测。结果 539例腹泻标本中,检出病毒阳性212例,单种感染207例,双重感染5例。A组轮状病毒检出率为4.27%(23/539),B组轮状病毒检出率为0,C组轮状病毒检出率为0,诺如病毒Ⅱ型检出率为31.91%(172/539),诺如病毒Ⅰ型检出率为1.48%(8/539),星状病毒检出率为0.93%(5/539),扎如病毒检出率为1.29%(7/539),腺病毒检出率为0.37%(2/539)。除诺如病毒Ⅱ的检出率在年龄分组中差异有统计学意义(χ2=10.26,P<0.05)外,其余均无统计学意义。8种病毒的检出率在性别分组中差异均无统计学意义。结论上海市西南地区成人腹泻患者中,诺如病毒和轮状病毒为病毒感染的主要病原体。更多还原

【Abstract】 [Objective]To study the etiologic characteristics of enteric viruses in adult diarrhea in the southwest of Shanghai.[Methods]Viruses were detected with reverse transcription real-time quantitative polymerase chain reactions( qRT-PCR).[Results]A total of 212 strains of pathogenic viruses were isolated from 539 specimens,including 207 cases of single infection and5 cases of double infection. The detection rate of rotavirus A,rotavirus B and rotavirus C was 4. 27%( 23 /539),0 and 0,respectively. The detection rate of norovirusⅡ,noroviruⅠ,astrovirus,sapovirus and adenovirus was 31. 91%( 172 /539),1. 48%(8/539),0.93%(5/539),1.29%(7/539) and0.37%(2/539),respectively. There was significant difference in the detection rate of norovirusⅡ among different age groups( χ2= 10. 26,P < 0. 05),while the differences in the detection rate of other viruses were not significant. There were no significant differences in the detection rate of eight viruses between males and females.[Conclusion]Norovirus and rotavirus are the main pathogens in adult patients with diarrhea in the southwest of Shanghai.更多还原