Objective To undersyand the genetic characteristics of Enterovirus Type 71( EV 71) isolated from clinical specimens of children with hand- foot- mouth disease( HFMD) in Shaoxing city from 2012 to 2013. Methods RNA was extracted from specimens collected from stool,throat swab and vesicle from children with HFMD,and EV 71 were identified by reverse transcriptase polymerase chain reaction( RT- PCR). Ten positive samples were isolated and were amplified by segmented PCR,then,they were spliced into a full- lengt...