【摘要】 目的:探讨江西省汉族人群中芳香族抗癫痫药物所致皮肤不良反应与HLA-B*1502基因的相关性。方法:采用病例对照研究,一共收集样本46例,其中Stevens-Johnson综合征(SJS)组13例,轻微皮肤反应(MPE)组19例,对照组(包括耐受者和正常志愿者)14例。采用序列特异性引物聚合酶链反应(SSPPCR)技术检测HLA-B*1502的基因型。采用Fisher确切概率法检验分析HLA-B*1502与抗癫痫药物皮肤不良反应的相关性。结果:HLA-B*1502基因频率分别为SJS组100%(13/13),MPE组10.5%(2/19),对照组7.1%(1/14)。SJS组分别与MPE组和对照组比较差异具有统计学意义(P<0.05),MPE组与对照组比较差异无统计学意义(P<0.05)。结论:江西汉族人群中芳香族AEDs导致的SJS与HLA-B*1502基因具有强相关性,而MPE与之无相关性。建议江西汉族人群在采用AEDs治疗前应该进行HLA-B*1502基因型检测,阳性者应避免服用芳香族AEDs,此外还需警惕阴性者发生SJS等严重皮肤不良反应。更多还原

【Abstract】 Objective: To investigate the correlation between the aromatic antiepileptic drugs(AEDs) induced cutaneous adverse drug reactions(c ADRs) and HLA-B*1502 gene in Han population of Jiangxi province. Method: The case-control study was taken, A total of 46 cases of samples were collected, including 13 patients with Stevens-Johnson syndrome(SJS)(SJS group), 19 patients with mild skin reaction(MPE)(MPE group), and 14 tolerance and normal volunteers as controls(control group).The sequence-specific primers polymerase chain reaction(SSP-PCR) technique was used to detect HLA-B*1502 genotype. The Fisher’s exact test was used to analyze the correlation between HLAB*1502 and AEDs-c ADRs. Result: The gene frequencies of HLA-B*1502were 100%(13/13) in SJS group, 10.5%(2/19) in MPE group and 7.1%(1/14) in the control group. There were significant differences in the SJS group compared with MPE group and the control group(P<0.05), there was no statistically significant difference between the MPE group and the control group(P>0.05). Conclusion: There is a strong correlation between HLA-B*1502 gene and the aromatic AEDs-induced SJS,but the correlation between HLA-B*1502 gene and MPE has not been found in Han population of Jiangxi province. HLA-B*1502 gene type testing before initiating AEDs therapy is suggested for Han population of Jiangxi province. Those with positive HLA-B*1502 should avoid taking aromatic AEDs. Furthermore,physicians should also be vigilant about SCR,such as SJS,in those with negative HLA-B*1502.更多还原