Stabilizing the Bactericidal Activity of Hydrogen Peroxide:A Brandnew Function of Certain Chinese Herbs

【摘要】 Objective:To explore natural herbs to maintain the bactericidal activity of hydrogen peroxide（H₂O₂）.Methods:Eighteen extracts of Chinese herbs were prepared complying with the standard protocol.Each of the solutions was then mixed with 1%H₂O₂.The mixtures were handled with two approaches:autoclaved daily for one,two or three times;stored at room temperature from one through five years.Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration（MBC）and minimal inhibitory concentration（MIC）against Gram positive（Staphylococcus aureus,ATCC25923）and Gram negative（Escherichia coil,ATCC12421）bacteria.Results:While mixed with 1%H₂O₂,10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving,and 8 of them preserved such level of MBC value after stored at room temperature for three years.Some Chinese herbs,i.e.R.Scutellariae,R.Coptidis,R.Bupleuri,H.Epimedii,C.Phelledendri and F.Chrysanthemi,can significantly maintain the bactericidal activity of diluted H₂O₂.Conclusions:Certain Chinese herbs can effectively stabilize the bactericidal activity of H₂O₂ undergoing autoclave or long-term storage.This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H₂O₂ usage while its stability level is promoted.更多还原

【Abstract】 Objective:To explore natural herbs to maintain the bactericidal activity of hydrogen peroxide（H₂O₂）.Methods:Eighteen extracts of Chinese herbs were prepared complying with the standard protocol.Each of the solutions was then mixed with 1%H₂O₂.The mixtures were handled with two approaches:autoclaved daily for one,two or three times;stored at room temperature from one through five years.Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration（MBC）and minimal inhibitory concentration（MIC）against Gram positive（Staphylococcus aureus,ATCC25923）and Gram negative（Escherichia coil,ATCC12421）bacteria.Results:While mixed with 1%H₂O₂,10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving,and 8 of them preserved such level of MBC value after stored at room temperature for three years.Some Chinese herbs,i.e.R.Scutellariae,R.Coptidis,R.Bupleuri,H.Epimedii,C.Phelledendri and F.Chrysanthemi,can significantly maintain the bactericidal activity of diluted H₂O₂.Conclusions:Certain Chinese herbs can effectively stabilize the bactericidal activity of H₂O₂ undergoing autoclave or long-term storage.This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H₂O₂ usage while its stability level is promoted.更多还原