【摘要】 目的通过检测心房颤动(房颤)与窦性心律患者右心耳组织中microRNA-1、microRNA-133及microRNA-34a的表达差异,及其可能靶蛋白锚蛋白-B(Ankyrin-B)的表达变化,分析两者之间的关系,以从microRNAs调控水平研究房颤发生、发展的新机制。方法将第三军医大学新桥医院40例风湿性心瓣膜病行心瓣膜置换术患者分为房颤组[男8例,女12例;年龄(52.9±5.8)岁]和窦性心律组[男9例,女11例;年龄(52.4±6.2)岁]。采用逆转录-实时定量聚合酶链式反应(RTQ-PCR,Real-time quantitive PCR)法检测患者右心耳组织中microRNA-1、microRNA-133及microRNA-34a的表达,ΔΔCt法计算结果;石蜡切片免疫组织化学法检测组织中Ankyrin-B的表达;蛋白免疫印迹(Western Blotting)法检测组织中Ankyrin-B的表达变化。结果房颤组患者右心房组织中的microRNA-1表达较窦性心律组显著降低(0.559±0.252 vs.3.997±1.251;t=-21.455,P=0.000),microRNA-133的表达较窦性心律组显著降低(0.630±0.238 vs.5.514±1.549;t=24.133,P=0.000),而microRNA-34a的表达较窦性心律组增高(4.783±2.012 vs.1.350±0.638,t=12.596,P=0.000)。免疫组织化学法及Western Blotting均显示房颤组心房组织Ankyrin-B表达较窦性心律组明显降低,且差异有统计学意义(0.66±0.45 vs.1.09±0.42;t=-3.396,P=0.001)。结论 MicroRNA-34a可能通过调节Ankyrin-B蛋白的表达,从而参与心房颤动的发生、发展。更多还原

【Abstract】 Objective To investigate different expressions of microRNA-1(microR-1),microRNA-133(microR-133), microRNA-34a(microR-34a) and their possible target protein Ankyrin-B in right atrial appendage of patients with atrial fi brillation(AF) or sinus rhythm(SR),analyze their correlation,and explore novel mechanisms of the pathogenesis and development of AF in microRNA level. Methods Forty right atrial appendage samples of 40 patients with rheumatic heart disease who underwent heart valve replacement in Xinqiao Hospital,Third Military Medical University were included in this study. All the patients were divided into AF group including 8 males and 12 females with their age of 52.9±5.8 years and SR group including 9 males and 11 females with their age of 52.4±6.2 years. The expression of microR-1,microR-133 and microR-34a were examined with RTQ-PCR(real-time quantitative PCR) and calculated with delta delta Ct method. Different expressions of Ankyrin-B between AF and SR group were examined with immunohistochemistry and Western Blotting. Results MicroR-1 expression of right atrial appendage of AF group was signifi cantly lower than that of SR group(0.559±0.252 vs. 3.997±1.251,t =-21.455,P=0.000),microR-133 expression of AF group was signifi cantly lower than that of SR group(0.630±0.238 vs. 5.514±1.549,t =24.133,P=0.000),and microR-34a expression of AF group was signifi cantly higher than that of SR group(4.783±2.012 vs. 1.350±0.638,t =12.596,P=0.000). Immunohistochemistry and Western Blotting showed Ankyrin-B expression in right atrial appendage of AF group was signifi cantly lower than that of SR group(0.66±0.45 vs. 1.09±0.42,t =-3.396,P=0.001). Conclusion MicroR-34a may take part in the pathogenesis and development of AF by regulating the expression of Ankyrin-B.更多还原