目的探讨RKIP基因对胃癌细胞株SGC7901恶性生物学行为的影响。方法采用脂质体转染技术,将RKIP真核表达重组质粒pc DNA3.1(+)/RKIP和空载体质粒分别导入胃癌细胞系SGC7901,定量-反转录-PCR(q-RT-PCR)和Western blot分别检测RKIP m RNA及蛋白质表达,构建RKIP基因稳定表达的胃癌细胞系SGC7901。借助细胞生长曲线及集落形成实验观察胃癌细胞增殖的变化;划痕试验及Transwell实验检测胃癌细胞迁移能力和侵袭力的变化;流式细胞术检测RKIP表达对胃癌细胞的细胞周期和凋亡率的影响;并建立人胃癌细胞裸鼠皮下移植瘤模型,观察RKIP对胃癌细胞增殖和转移能力的影响。结果转染RKIP基因的SGC7901细胞系RKIP m RNA和蛋白质表达量显著增加。转染组SGC7901细胞较对照组生长速度减慢,集落形成率显著下降,流式细胞术分析发现,RKIP延缓细胞由G0~G1期进入S期,诱导细胞凋亡,裸鼠接种实验显示,RKIP基因可使裸鼠成瘤平均时间延长,生成移植瘤体积显著减小。结论 RKIP与胃癌的增殖、凋亡和侵袭等生物学行为密切相关,RKIP基因重表达有助于SGC790...
【英文摘要】
【Objective】To determine the effects of RKIP gene on biological charateristics of gastric carcinoma cell line SGC7901.【Methods】The RKIP gene eukaryotic expression vector pc DNA3.1(+)-RKIP was transfected into SGC7901 cells by lipid media transfection. Cells untransfected SGC7901 cells and those transfected with pc DNA3.1(+) plasmid were used as controls. The expression levels of RKIP m RNA and protein in SGC7901 cells were detected by q-RT-PCR and Western blotting analysis, respectively. The proliferative ac...
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