【摘要】 目的血管内皮生长因子(vascular endothelial growth factor,VEGF)与肿瘤血管生成、生长和转移密切相关,该基因的多态位点可能影响基因的表达从而影响肿瘤的发生和发展,本研究采用病例-对照的方法,探讨VEGF基因多态性与肺癌发病风险的关系。方法采用聚合酶链反应-限制性片段长度多态性技术检测300例肺癌患者和300例对照人群VEGF基因-2578C/A多态性位点基因型和等位基因频率分布情况。结果 VEGF-2578C/A位点,C/C、C/A、A/A三个基因型在肺癌组和对照组中的分布频率分别为58.3%、34.7%、7.0%和69.7%、25.0%、5.3%,两组相比差异有显著性(χ2=8.384,P=0.015),肺癌组中A等位基因型的频率为25.3%显著高于对照组17.8%(P=0.006),与C/C基因型相比,携带A(C/A+A/A)等位基因型能明显增加肺癌的发病风险(OR=1.644,95%CI=1.168~2.314);以吸烟状况分层分析,携带A等位基因型能明显增加吸烟人群中肺癌的发病风险(OR=2.017,95%CI=1.218~3.342);以病理类型分层分析发现,C/C、C/A、A/A基因型在鳞癌患者组中的频率为50.5%、40.7%和8.8%,与对照组相比差异有显著性(P=0.006),携带A等位基因型能明显增加鳞癌的发病风险(OR=2.172,95%CI=1.344~3.508)。结论本研究结果提示VEGF-2578A等位基因型能明显增加肺癌的发病风险,并且与肺癌的病理类型有关。更多还原

【Abstract】 【Objective】Angiogenesis is an essential process in the development,growth,and metastasis of malignant tumors. Polymorphisms in the vascular endothelial growth factor(VEGF) gene may lead to altered VEGF production and/or activity,thereby causing interindividual differences in the susceptibility to lung cancer via their actions on the pathways of tumor angiogenesis. This study was designed to investigate the correlation of-2578C/A SNPs with the risk of Lung Cancer.【Methods】Genotypes were analyzed by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) assay method in 377 patients with lung cancer and 416 matched healthy controls. 【Results】The 2578C/A SNP,C/C C/A A/A genotpye frequencies in patients and controls group were 58.3%,34.7%,7.0%and 69.7%,25.0%,5.3% respectively. There was a significant difference between the two group(χ2=8.384,P = 0.015). The frequency of allele A(25.3%) was significantly higher than the healthy controls 17.8%(P =0.006). Compared with the C/C genotype,the allele A(C/A+A/A) could significantly increase the risk of lung cancer,(OR=1.644,95%CI=1.168~2.314). Stratified analysis on smoking condition and A type alleles carrierscan significantly increase the risk of lung cancer in smokers(OR=2.017,95%CI=1.218~3.342).Stratification analysis showed that the C/C C/A A/A genotpye frequencies in squamouscell carcinoma were 50.5%,40.7%,8.8% with significant differencecompared with the control groups. A significant increase in lung cancer risk was seen among squamouscell carcinoma groups if they carried at least one A allele(OR=2.172,95%CI=1.344~3.508) compared to control individuals without carrying these genotype.【Conclusion】Our results indicated that VEGF-2578A allele could significantly increase the risk of lung cancer,played different roles in different pathological type.更多还原