The leaf from sterile seedling of Scrophularia buergeriana was acted as explants, directly induced adventitious buds and got the regenerated plant. In vitro regeneration of rapid propagation system by tissue culture was established. The results showed that immersed with 0.1% Hg Cl2 for 6 min was the best disinfection method for S. buergeriana seeds with the contamination rate of 4.95% and the sprout rate was 81.49%; the best medium for adventitious buds induction was MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1, the i...