【摘要】 目的了解抗艾滋病病毒治疗患儿病毒学失败者的耐药突变特点。方法采集接受过高效抗病毒治疗患儿的血浆,对病毒载量≥1 000拷贝/ml者进行耐药基因型检测,将所得序列上传到斯坦福大学耐药数据库(http://hivdb.stanford.edu)进行对比分析。结果发生病毒学失败的有66例患儿,其中38例患儿出现了耐药突变,耐药发生率为57.58%(38/66)。逆转录酶抑制剂的耐药发生率(56.06%,37/66)明显高于蛋白酶抑制剂的耐药发生率(3.03%,2/66)。结论 HIV-1耐药突变是导致治疗失败的主要原因,在艾滋患儿开始抗病毒治疗之前进行基因型耐药检测是必要的,可为制定适用的抗病毒治疗方案提供数据。更多还原

【Abstract】 Objective To assess the prevalence and the characteristic of HIV-1 drug resistance among children with virological failure after highly active anti-retroviral therapy( HAART). Methods Plasma samples were collected from children whose viral load were more than 1 000 copies / ml,then the detection of HIV drug resistance was carried out. The sequences’ phylogenesis were analyzed after a comparison by landing the websites( http: / / Hivdb. stanford. edu). Results 38 cases exhibited the resistance to antiretroviral drugs among 66 children with virological failure. The rate of the drug-resistance was 57. 58%( 38 /66). The rate of resistance to RTs( 56. 06%,37 /66) was much higher than PIs( 3. 03%,2 /66). Conclusion The main causation which resulted in virological failure was drug resistance. The detection of HIV drug resistance ahead of HAART was useful so that we can make the better plan of ART.更多还原