【摘要】 为筛选有效防治罹病柑橘植株体内黄龙病菌的药剂,选取6种药剂进行韧皮部输液,利用实时定量PCR对病株体内带菌量进行动态监测以确定相对防效。结果表明,连续施药8个月后,硫酸铜、布罗波尔处理组和对照组带菌量下降了20%~30%,2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮以及氨苄青霉素带菌量减少了80%以上;ZnSO4处理带菌量下降了65.60%。各观察期内相对防效都超过50%的药剂有2,2-二溴-3-次氮基丙酰胺(≥50%)和氨苄青霉素(≥70%)。相对防效分析表明,施药后8个月1g/L 2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮、氨苄青霉素相对防效分别达到了89.75%、83.72%和87.27%,初步判断这3种药剂对黄龙病病树有防控效果。更多还原

【Abstract】 In order to screen effective chemical compounds against the citrus Huanglongbing(HLB)bacterium Candidatus Liberibacter asiaticus in citrus plants,6 chemical compounds were injected into phloem.HLB bacterial titers were dynamically measured by real-time quantitative PCR to determine the control effect.The results showed that,after eight months of continuous injection,the bacterial titers of copper sulfate,bronopol and the control dropped by 20%-30% versus a 65.60% decline in ZnSO4treatment,while 2,2-dibromo-3-nitrilopropionamide(DBNPA),isothiazolin-ketone and ampicillin achieved more than 80% decrease of bacterial titers.The control effects all reached more than50% by both treatments of DBNPA(≥50%)and ampicillin(≥70%)during each observation period over eight months.The control efficiency analysis demonstrated that the control effects of DBNPA,isothiazolin-ketone and ampicillin were 89.75%,83.72% and 87.27%,respectively,at the same dosage of 1 g/L after eight months of initial treatment.This preliminary study indicated that these three chemical compounds suppressed the Ca L.asiaticus in HLB-affected citrus plants.更多还原