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柑橘黄龙病防控药剂筛选试验初报
Preliminary report on screening of chemical compounds against the citrus Huanglongbing bacterium Candidatus Liberibacter asiaticus in citrus plants
【摘要】 为筛选有效防治罹病柑橘植株体内黄龙病菌的药剂,选取6种药剂进行韧皮部输液,利用实时定量PCR对病株体内带菌量进行动态监测以确定相对防效。结果表明,连续施药8个月后,硫酸铜、布罗波尔处理组和对照组带菌量下降了20%~30%,2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮以及氨苄青霉素带菌量减少了80%以上;ZnSO4处理带菌量下降了65.60%。各观察期内相对防效都超过50%的药剂有2,2-二溴-3-次氮基丙酰胺(≥50%)和氨苄青霉素(≥70%)。相对防效分析表明,施药后8个月1g/L 2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮、氨苄青霉素相对防效分别达到了89.75%、83.72%和87.27%,初步判断这3种药剂对黄龙病病树有防控效果。
【Abstract】 In order to screen effective chemical compounds against the citrus Huanglongbing(HLB)bacterium Candidatus Liberibacter asiaticus in citrus plants,6 chemical compounds were injected into phloem.HLB bacterial titers were dynamically measured by real-time quantitative PCR to determine the control effect.The results showed that,after eight months of continuous injection,the bacterial titers of copper sulfate,bronopol and the control dropped by 20%-30% versus a 65.60% decline in ZnSO4treatment,while 2,2-dibromo-3-nitrilopropionamide(DBNPA),isothiazolin-ketone and ampicillin achieved more than 80% decrease of bacterial titers.The control effects all reached more than50% by both treatments of DBNPA(≥50%)and ampicillin(≥70%)during each observation period over eight months.The control efficiency analysis demonstrated that the control effects of DBNPA,isothiazolin-ketone and ampicillin were 89.75%,83.72% and 87.27%,respectively,at the same dosage of 1 g/L after eight months of initial treatment.This preliminary study indicated that these three chemical compounds suppressed the Ca L.asiaticus in HLB-affected citrus plants.
【Key words】 Huanglongbing; phloem; 2,2-dibromo-3-nitrilopropionamide; isothiazolin-ketone; ampicillin;
- 【文献出处】 植物保护 ,Plant Protection , 编辑部邮箱 ,2014年02期
- 【分类号】S436.661
- 【被引频次】19
- 【下载频次】474