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柑橘黄龙病防控药剂筛选试验初报

Preliminary report on screening of chemical compounds against the citrus Huanglongbing bacterium Candidatus Liberibacter asiaticus in citrus plants

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【作者】 陈仕钦卢小林陈玉龙吴东杨必赛王中康

【Author】 Chen Shiqin;Lu Xiaolin;Chen Yulong;Wu Dong;Yang Bisai;Wang Zhongkang;College of Life Sciences,Chongqing University;Lucky Team Biotech Development(Hepu)Ltd.,Co.;

【机构】 重庆大学生命科学学院亚洲果业广西利添生物科技发展有限公司

【摘要】 为筛选有效防治罹病柑橘植株体内黄龙病菌的药剂,选取6种药剂进行韧皮部输液,利用实时定量PCR对病株体内带菌量进行动态监测以确定相对防效。结果表明,连续施药8个月后,硫酸铜、布罗波尔处理组和对照组带菌量下降了20%~30%,2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮以及氨苄青霉素带菌量减少了80%以上;ZnSO4处理带菌量下降了65.60%。各观察期内相对防效都超过50%的药剂有2,2-二溴-3-次氮基丙酰胺(≥50%)和氨苄青霉素(≥70%)。相对防效分析表明,施药后8个月1g/L 2,2-二溴-3-次氮基丙酰胺、异噻唑啉酮、氨苄青霉素相对防效分别达到了89.75%、83.72%和87.27%,初步判断这3种药剂对黄龙病病树有防控效果。

【Abstract】 In order to screen effective chemical compounds against the citrus Huanglongbing(HLB)bacterium Candidatus Liberibacter asiaticus in citrus plants,6 chemical compounds were injected into phloem.HLB bacterial titers were dynamically measured by real-time quantitative PCR to determine the control effect.The results showed that,after eight months of continuous injection,the bacterial titers of copper sulfate,bronopol and the control dropped by 20%-30% versus a 65.60% decline in ZnSO4treatment,while 2,2-dibromo-3-nitrilopropionamide(DBNPA),isothiazolin-ketone and ampicillin achieved more than 80% decrease of bacterial titers.The control effects all reached more than50% by both treatments of DBNPA(≥50%)and ampicillin(≥70%)during each observation period over eight months.The control efficiency analysis demonstrated that the control effects of DBNPA,isothiazolin-ketone and ampicillin were 89.75%,83.72% and 87.27%,respectively,at the same dosage of 1 g/L after eight months of initial treatment.This preliminary study indicated that these three chemical compounds suppressed the Ca L.asiaticus in HLB-affected citrus plants.

【基金】 公益性行业(农业)科研专项(201003067);中央高校基本科研业务费专项资金(CDJXS112300)
  • 【分类号】S436.661
  • 【被引频次】19
  • 【下载频次】474
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