【摘要】 目的体外探索促红细胞生成素(EPO)拮抗环孢素A(CsA)所致肾小管上皮损伤的作用机制。方法采用人近曲肾小管上皮细胞作为体外实验模型,实验分对照组、CsA对照组、EPO 15U/mL与CsA合用组、EPO 30U/mL与CsA合用组,MTT法检测细胞增殖抑制率,采用金氏公式评价EPO与CsA合用拮抗作用效果,采用ELISA方法检测细胞培养上清液中肾损伤分子-1(KIM-1)的表达量,采用流式细胞仪检测细胞生存情况。结果采用金氏公式评价结果显示,EPO在15U/mL与30U/mL浓度时可以拮抗CsA对肾小管上皮的损伤作用;对比CsA对照组,EPO在15U/mL与30U/mL浓度可降低细胞培养上清液中KIM-1的表达量(P<0.05),降低凋亡细胞比率(P<0.05),降低坏死细胞比率(P<0.05),增加活细胞比率(P<0.05)。结论 EPO拮抗CsA所致肾小管上皮损伤,可能通过减少细胞凋亡而实现。更多还原

【Abstract】 Objective To investigate the effect of Erythropoietin(EPO)on the human renal tubular cells injured by cyclosporin A(CsA)and its mechanism.Methods human renal proximal tubular epithelial cells was used as in vitro model,experiments were divided into control group,CsA group,EPO group,15U/mL combined with CsA EPO 30U/mL combined with CsA group,the cell proliferation was detected by MTT inhibition rate,using EPO and CsA’s formula evaluation combined with antagonistic effect,detection of cell culture supernatant of kidney injury molecule in-1by using the method of ELISA(KIM-1)expression,the cell viability was detected by flow cytometry.Results the Jin’s formula evaluation results show that,EPO can antagonize the effect of CsA on the injury of renal tubular epithelium in 15U/mL and 30U/mL concentration;compared with CsA group,EPO could decrease the expression of KIM-1in the supernatants of cells cultured in 15 U/mL and 30 U/mL concentration(P<0.05),decrease apoptotic cell ratio(P<0.05),reduce the ratio of necrotic cells(P<0.05),increasing the ratio of living cells(P<0.05).Conclusion EPO antagonized the CsA induced renal tubular epithelial injury,may be achieved by reducing cell apoptosis.更多还原