【摘要】 目的探讨糖尿病自身抗体联合检测在糖尿病分型中的应用价值,为正确分型糖尿病提供理论依据。方法选择我院内分泌科收治的126例糖尿病患者,其中1型糖尿病组51例,2型糖尿病组75例,同时选择健康对照组60例,采用免疫印迹试剂法检测胰岛细胞抗体(ICA)、胰岛素自身抗体(IAA)和谷氨酸脱羧酶抗体(GADA)表达情况,计算阳性率,并对结果进行比较分析。结果 ICA、IAA和GADA在1型糖尿病患者中的阳性率分别为35.3%、31.4%和60.8%,显著高于2型糖尿病患者的1.3%、1.3%、2.7%及对照组的1.3%、0.0%、0.0%,均有显著性差异(P<0.05)。ICA/IAA/GADA、GADA/ICA、GADA/IAA三种联合检测组合对1型糖尿病诊断的敏感性分别为92.2%、84.3%和82.4%,均显著高于单一抗体检测,均有显著性差异(P<0.05)。结论检测血清ICA、IAA和GADA能够为正确鉴别1型糖尿病和2型糖尿病提供重要依据,联合检测能够提高诊断阳性率,避免漏诊,值得临床推广和普及。更多还原

【Abstract】 Objective To explore the diagnostic value of detection of diabetes antibodies in the classification of diabetes mellitus and provide a theoretical basis for the correct typing of diabetes mellitus.Methods 126 cases of diabetic patients were hospitalized in the Department of Endocrinology,including 51cases of type 1 diabetes,75 cases of type 2 diabetes,and 60 healthy controls were detected as control group.Islet cell antibodies(ICA),insulin autoantibody(IAA),glutamic acid decarboxylase antibody(GADA)expression and the positive rate were tested.Results The positive rates of ICA,IAA,GADA in patients with type 1 diabetes were 35.3%,31.4%,60.8%,were significantly higher than 1.3%,1.3%,2.7% and 1.3%in patients with type 2 diabetes(P<0.05).The sensitivity of combined detection of antibodies ICA/IAA/GADA,GADA/ICA and GADA/IAA in the diagnosis of type 1diabetes were 92.2%,84.3%,82.4%,were significantly higher than those of single detection(P<0.05).Conclusion The detection of serum ICA,IAA,GADA can provide an important basis for the correct identification of type 1diabetes and type 2diabetes.Joint detection can improve the positive rate of diagnosis and avoid misdiagnosis,is worth the clinical promotion and popularization.更多还原