【摘要】 目的检测深圳市宫颈癌患者癌组织中HPV16型E6、E7致癌基因的序列。方法采用PCR技术扩增1例在深圳生活17年宫颈癌HPV16型阳性患者组织中HPV16E6、E7基因,对其进行克隆、构建重组质粒并进行核酸限制性内切酶鉴定及DNA测序分析。结果成功地克隆了HPV16E6、E7基因,测序分析表明克隆的HPV16E6、E7基因与GenBank收录的德国标准株相比完全一致。结论深圳市妇女宫颈癌组织中HPV16型E6、E7致癌基因的序列与德国标准株相同。更多还原

【Abstract】 Objective To investigate the coding sequences of HPV16E6/E7gene extracted from the cervical cancer tissues from patients in Shenzhen.Methods The HPV16E6/E7gene was extracted from a HPV16positive cervical cancer patient that had been living in Shenzhen for 17years.The gene was amplified by PCR and cloned;the recombinant plasmid was constructed;and the DNA Sequence was restricted by restriction endonuclease.Results The HPV16 E6/E7gene was successfully cloned and the sequencing analysis showed that the cloned HPV16E6/E7gene is identical to the German standard strain documented in GenBank.Conclusion The coding of HPV16E6/E7gene extracted from cervical cancer tissues from female patient in Shenzhen is identical to the German standard strain.更多还原