【摘要】 为测定鸡传染性法氏囊病活疫苗(B87株)的病毒含量,采用Vero细胞建立鸡传染性法氏囊病活疫苗(B87株)病毒含量细胞间接免疫荧光方法,测定细胞半数感染量(TCID50)。通过与传统鸡胚半数致死量(ELD50)方法进行敏感性和相关性比较,间接免疫荧光方法具有更高的敏感性,两种方法的相关系数r为0.823。间接免疫荧光方法的建立为该疫苗的检验提供了一种新途径。更多还原

【Abstract】 The Vero cell was used to establish a cellular immunofluorescence method for evaluation of living infectious bursal disease vaccine( B87 strain). Use the method to determine the amount of virus of the vaccine( TCID 50), compared with traditional method( ELD 50),and the correlation coefficient r =0.823. If the method is used to evaluate the vaccine,it will reduce inspection costs,shorten the inspection cycle,and improve test sensitivity.更多还原