【摘要】 10日龄的健康艾维茵肉仔鸡250只,随机分为5组。21日龄时除阴性对照组(Ⅳ组)外均用新城疫病毒(Newcastle disease,virus,NDV)强毒株攻毒,各治疗组(Ⅰ~Ⅲ组)在22~25日龄饲料中分别添加0.5%、1.0%、2.0%的复方中药"禽康散",第Ⅳ、Ⅴ组(阳性对照组)不用药。在攻毒前1d和攻毒后1、3(用药后2d)、7(停药后2d)、15(停药后10d)d观察血清免疫球蛋白及新城疫抗体效价的变化。结果显示:Ⅰ、Ⅱ组血清IgG在攻毒15d(停药10d)时与Ⅲ、Ⅳ、Ⅴ组差异极显著(P<0.01);Ⅰ~Ⅲ组血清IgA浓度在攻毒后3、7d均明显高于第Ⅴ组(P<0.05或0.01),以1%治疗组效果最佳;各组之间血清中IgM含量无显著差异(P>0.05)。Ⅰ~Ⅲ组新城疫抗体效价在攻毒7d后各阶段均高于阳性对照组(P<0.05或0.01),以第Ⅲ组最佳。结果表明,日粮添加1.0%~2.0%质量分数的"禽康散"能明显增加血清中IgG、IgA含量和新城疫抗体效价,从而提高机体体液免疫功能,达到提高机体抗NDV的能力。更多还原

【Abstract】 Two hundred and fifty broilers of 10-day-old were divided randomly into 5groups treated with virus and 0.5%(Ⅰ),1.0%(Ⅱ),2.0%(Ⅲ),negative control group(Ⅳ)and positive control group(Ⅴ).All groups exceptⅣ group were inoculated NDV-F48E9at 21-day-old,then treatment groups were given drug(Qinkangsan)at 0.5%,1.0%and 2.0%in the forage at 22to25-day-old.Blood immune globulin and ND-HI immune globulin of broilers were observed at 1d before and 1,3,7and 15dafter inoculation.Test result:IgG ofⅠ,Ⅱgroup was remarkably higher than that ofⅢ,ⅣandⅤgroup at 15dafter inoculation(P<0.01).IgA ofⅠ,ⅡandⅢ group was higher than that ofⅤgroup at 3,7dafter inoculation(P<0.05or 0.01),IgA ofⅡgroup was the highes.IgM of all groups were no remarkable difference.ND-HI immune globulin ofⅠ,ⅡandⅢ group was higher than that ofⅤgroup,Ⅱgroup was the highest.The result indicated that1.0%and 2.0%complex Chinese crude drug"Qinkangsan"in the forage could raise humor immune function by increase of IgG,IgA and ND-HI immune globulin,thus build up broilers’ s resistance to NDV.更多还原