【摘要】 研究了CO2超临界流体萃取糖蜜酒精废液中黄酮类化合物的影响因素,在单因素试验的基础上,以总黄酮提取率为主要评价指标,进行了L9(34)正交优化。结果表明,通过单因素实验,选择甲醇为夹带剂,确定萃取体系的温度、压力、时间及夹带剂流量为正交实验的主因素和取值范围。正交实验得最佳工艺条件为:萃取体系温度50℃、萃取时间120min、萃取压力300Bar(1Bar=0.1MPa)、甲醇夹带剂流量0.3mL/min,此条件下试验超临界二氧化碳萃取的黄酮类化合物的总量为0.289%,对DPPH自由基清除率为85.2%,确定了其中一种物质为4’,5,7-三羟基-3’,5’-二甲氧基异黄酮。由方差分析可知温度对萃取黄酮类化合物总量的影响达极显著水平,各因素影响顺序为萃取温度>萃取时间>萃取压力>夹带剂用量。更多还原

【Abstract】 The effects of the extraction parameters on the yield of flavonoids from molasses vinasse were studied. The process parameters were optimized by L9( 34) orthogonal tests based on single factor tests with the yield of total flavonoids as index. The optimal process parameters were: 50℃,300 Bar( 1Bar = 0. 1MPa),120min and 0. 3mL /min. The maximum extraction yield of flavonoids compounds was 0. 289%. The flavonoids had 85. 2% scavenging effect on DPPH. One of the flavonoid compounds was identified as 5,7,4’- trihydroxy- 3’; 5’- dimethoxyflavone. The results showed that the order of the extraction parameters affecting the yield of flavonoids is as follows: temperature > time >pressure > cosolvent amount.更多还原