【摘要】 目的研究2005—2013年河北省即食食品中单增李斯特菌污染状况及耐药特征。方法单增李斯特菌检验参照(GB/T 4789.30),药物敏感性试验应用微量肉汤稀释法,对15种抗生素进行耐药检测。结果单增李斯特菌总污染率为1.34%,凉拌菜及沙拉(2.62%)、生食水产品(2.51%)的检出率均高于所测其他食品。136株单增李斯特菌对15种抗生素耐药率为13.97%,以氯霉素耐药率最高(7.35%),其次是四环素和复方新诺明(均为4.41%),强力霉素和环丙沙星的耐药率均为2.94%,所有菌株对青霉素、亚胺培南、头孢噻吩、利福平、氨苄西林-舒巴坦酸敏感。结论河北省即食食品中存在单增李斯特菌污染,分离菌株存在较严重的耐药情况,且耐药性有增长趋势,应加强即食食品的监管以保证食品安全及公众健康。更多还原

【Abstract】 Objective To evaluate the prevalence and antimicrobial resistances of Listeria monocytogenes in ready-to-eat foods in Hebei Province from 2005 to 2013. Methods The detection of L. monocytogenes was referred to GB / T 4789. 30.The antimicrobial susceptibility was determined by broth microdilution susceptibility testing. Results Total prevalence of L. monocytogenes was 1. 34% in ready-to-eat foods,in which the higher prevalence in cold dish or salad was 2. 62%,and2. 51% in raw aquatic products. Among 15 antimicrobials,13. 97% strains resistant to one or more antibiotics. The resistant rate of chloramphenicol,tetracycline and sulfamethoxazole,doxycycline and ciprofloxacin were the top three,which were 7. 35%,4. 41% and 2. 94%,respectively. All strains were sensitive to penicillin,imipenem,cefalotin,rifampicin and ampicillin-sulbactam. Conclusion There was a certain degree of L. monocytogenes contamination in readyto-eat foods in Hebei Province. It was suggested that the antimicrobial resistance was serious and grow rapidly. More attention should be paid to the supervision of ready-to-eat foods to ensure food safety and public health.更多还原