【摘要】 目的建立了淀粉类食品中姜黄色素与类胡萝卜素化合物的高效液相色谱分析方法。方法采用环已烷-乙酸乙酯二元体系提取试样中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素、叶黄素和β-胡萝卜素,硅胶SPE柱净化,YMCTMCarotenoid色谱柱(250 mm×4.6 mm,5μm)分离,甲基叔丁基醚和甲醇-水梯度洗脱。对所分析化合物的稳定性、前处理条件及色谱条件进行了探讨与优化。结果 5种分析物在0.5~100μg/ml范围内线性关系良好,相关系数为0.998 4~0.999 8。在1~100 mg/kg浓度范围内,平均加标回收率为68.5%~101.2%,相对标准偏差为3.4%~10.4%。定量限为1 mg/kg。结论本方法稳定、可靠,可应用于实际样品的检测。更多还原

【Abstract】 Objective An effective high-performance liquid chromatography( HPLC) method was developed for the determination of curcumin,demethoxycurcumin,bisdemethoxycurcumin,β-carotenoid and lutein in starchy foods,and could be applied to the routine work. Methods The compounds were extracted from homogenized samples using cyclohexane-ethyl acetate( 80 ∶ 20,V / V) and cleaned up with silica gel SPE cartridges,then separated by a YMCTM carotenoid column( 250 mm × 4. 6 mm,5 μm). The influence of extraction solvents and mobile phase on resolution was investigated. Results The method was validated with respect to precision,accuracy,and linearity. The linear range was0. 5-100 μg / ml in spiked samples and the coefficients were 0. 998 4-0. 999 8. The average recoveries and the relative standard deviation ranged from 68. 5%-101. 2% and 3. 4%-10. 4%, respectively, for the spiked levels ranging at1-100 mg / kg for each compound. The limits of quantification( LOQ) of all compounds were 1 mg / kg. Conclusion This method was suitable for the determination of curcuminoides and carotenoids in starchy food.更多还原