Objective: To develop a method of high performance liquid chromatography coupled with a photodiode array detector( HPLC-DAD) for the content determination of six active flavonoids( quercetin-3-Osambubioside, rutin and quercetin-3-O-β-D-glucopyranside, kaempferol-3-O-β-D-glucopyranside, quercetin,kaempferol) in Oldenlandia diffusa. Method: The analyses were performed on a Diamonsil-C18column( 4. 6 mm× 250 mm,5 μm) eluted with acetonitrile and 0. 05% phosphoric acid in gradient mode. The flow rate was 1 mL·mi...