【摘要】 目的:测定楤木总皂苷在水,0.1 mol·L-1盐酸和不同pH磷酸盐缓冲液中的平衡溶解度,为楤木总皂苷提取物的剂型设计提供实验依据。方法:以楤木皂苷A为指标,采用HPLC测定楤木皂苷A在不同介质中平衡溶解度,流动相乙腈(A)-0.1%磷酸溶液(B)梯度洗脱(0~18 min,35%~43%A;18~19 min,43%A;19~25 min,43%~35%A),检测波长205 nm。结果:37℃时,楤木皂苷A在水,0.1 mol·L-1盐酸和pH 2,4,6,7,8,10的磷酸盐缓冲液中平衡溶解度分别为1.780,0.241,0.420,0.472,1.196,1.746,0.180,0.242 g·L-1。结论:楤木总皂苷提取物微溶于水,但受介质的pH影响大。更多还原

【Abstract】 Objective: To establish a method for determination equilibrium solubilities of total saponins extract from Aralia chinensis in different mediums. Method: Taking araloside A as index,an HPLC method was performed and adopted to detect the concentration of araloside A in water,0. 1 mol ·L-1HCl and different phosphate buffer with pH of 2,4,6,7,8,10 at 37 ℃,mobile phase of acetonitrile( A)-0. 1% phosphoric acid solution( B) for gradient elution( 0-18 min,35%-43% A; 18-19 min,43% A; 19-25 min,43%-35%A),detection wavelength was set at 205 nm. Result: Equilibrium solubilities of araloside A in different mediums were 1. 780,0. 241,0. 420,0. 472,1. 196,1. 746,0. 180,0. 242 g ·L-1. Conclusion: Total saponins extract from A. chinensis dissolves in water,its equilibrium solubility is influenced greatly by pH values.更多还原