【摘要】 目的:制备蛇鳞草-大黄固体脂质纳米粒并考察其形态。方法:采用HPLC测定三羽新月蕨苷A和大黄素含量,流动相乙腈(A)-0.1%甲酸水溶液(B)-水(C)梯度洗脱(0~35 min,15%~75%A,25%B,60%~0%C),检测波长226 nm。利用薄膜超声法制备蛇鳞草-大黄固体脂质纳米粒,在单因素试验基础上,以三羽新月蕨苷A和大黄素包封率为综合评价指标,采用正交试验优选处方。结果:最佳处方为加药量150μL,硬脂酸-卵磷脂质量比1∶2,吐温-80质量分数1%;纳米粒平均粒径(301±2.8)nm,Zeta电位(-19.94±3.82)mV,三羽新月蕨苷A和大黄素包封率分别为61.8%和81.2%。结论:制备的蛇鳞草-大黄固体脂质纳米粒基本呈规则球形或类球形,该工艺稳定可行。更多还原

【Abstract】 Objective: To prepare Prpnephrium triphyllum-Rheum officinale solid lipid nanoparticles and investigate its morphology. Method: HPLC was adopted to determine contents of emodin and triphyllin A with mobile phase of acetonitrile-0. 1% formic acid-water for gradient elution and detection wavelength at 226 nm.Film-ultrasonic wave dispersion technique was used to prepare P. triphyllum-R. officinale solid lipid nanoparticles,on the basis of single factor tests,orthogonal test was employed to optimize preparation technique by taking entrapment efficiencies of emodin and triphyllin A as comprehensive evaluation index. Result: Optimal formulation was as following: extract of P. triphyllum-R. officinale 150 μL,stearic acid-soybean lecithin( 1∶ 1),tween-80 of 1. 0%; mean particle size diameter was( 301 ± 2. 8) nm,Zeta potential was(-19. 94 ± 3. 82) mV,entrapment efficiencies of triphyllin A and emodin was 61. 8% and 81. 2%,respectively. Conclusion: These prepared P. triphyllum-R. officinale solid lipid nanoparticles show spherical,this preparation process is stable and feasible.更多还原