【摘要】 目的:建立气相色谱法测定不同产地丝瓜子脂肪油中4种脂肪酸含量的方法,为丝瓜子质量评价和标准制定提供实验依据。方法:采用HP-FFAP毛细管色谱柱(0.2 mm×50 m,0.3μm),载气高纯度氮气,流速0.5 mL·min-1,分流比23∶1,柱温235℃,进样口温度250℃,初始温80℃,FID检测器,温度250℃,空气流量450 mL·min-1,氢气流量45 mL·min-1。结果:丝瓜子中棕榈酸质量分数为2.325%~2.985%;硬脂酸质量分数为1.198%~1.771%,油酸质量分数为2.212%~5.159%,亚油酸质量分数为7.383%~13.578%。结论:该方法准确、可靠、重复性好,可用于丝瓜子脂肪油中亚油酸、油酸、棕榈酸、硬脂酸的质量控制。更多还原

【Abstract】 Objective: To establish a method for the determination of the content of four kinds of fatty acids in Luffa cylindrica seed by GC,and provide evidence for evaluating quality and criterion. Method: The GC separation was performed on a HP-FFAP column( 0. 2 mm × 50 m,0. 3 μm) at 235 ℃,inject temperature at 250℃,FID detecter temperature was 250 ℃. Gas as N2( 99.99%); the flow rate was 0.5 mL·min- 1; split ratio23∶ 1. Result: The content of palmtinic acid in L. cylindrica seed were 2. 325%-2. 985%,octadecanoic acid1. 198%-1. 771%,oleic acid 2. 212%-5. 159%,linoleic acid 7. 383%-13. 578%. Conclusion: The method can be used to control the quality of four kinds fatty acids in of L. cylindrica seed,which is accurate,convenient and repeatable.更多还原