【摘要】 目的:采用高效液相色谱法建立丹七片的指纹图谱。方法:资生堂CAPCELL PARK C18色谱柱(4.6 mm×250mm,5μm),流动相乙腈-0.06%磷酸溶液(梯度洗脱),检测波长210 nm,柱温30℃,流速为1.0 mL·min-1。采用"中药色谱指纹图谱相似度评价系统(2004A)"对10批丹七片指纹图谱进行分析。结果:10批丹七片的HPLC指纹图谱有15个共有峰,确认了其中7个色谱峰的成分分别为丹参素钠、原儿茶醛、迷迭香酸、紫草酸、人参皂苷Rg1、丹酚酸B和人参皂苷Rb1,相似度>0.990。结论:该法操作简便,精密度、重复性和稳定性良好,为丹七片的质量控制提供了有效手段。更多还原

【Abstract】 Objective: To establish HPLC fingerprint of Danqi Tablets. Method: The separation was performed on Shiseido CAPCELL PARK C18(4. 6 mm × 250 mm,5μm) column with mobile phase of acetonitrile-0. 06% phosphate(gradient elution) at flow rate of 1. 0 mL·min-1. Column temperature was 30 ℃ and detection wavelength was set at 210 nm. Fingerprint of 10 batches of Danqi tablet was analysed by 2004A edition of chromatographic fingerprint similarity evaluation system Result: Fifteen common peaks were selected as the fingerprint peaks. from 10 batches of Danqi tablet and seven of these common peaks were identified as Salvianic acid A sodium,protocatechualdehyde,rosmarinic acid,alkannic acid,ginsenoside-Rg1,salvianolic acid B and ginsenoside-Rb1. Conclusion: The method established is simple,selective,accurate and reproducible,which can be used for the quality control of Danqi tablets.更多还原